Current TFLEx member agencies:
- Alameda-Contra Costa Transit District – Oakland, Fremont, Berkeley
- Bay Area Rapid Transit Office of Inspector General – San Francisco
- Bi-State Development Agency – St. Louis
- C TRAN – Vancouver
- Capital Area Transit – Baton Rouge
- Central Ohio Transit Authority – Columbus
- Central Puget Sound Regional Transit Authority – Seattle
- Culver City Bus – Culver City
- Dallas Area Rapid Transit – Dallas
- Denton County Transportation Authority – Denton, Highland Village, Lewisville
- Denver Regional Transportation District – Denver
- Foothill Transit – San Gabriel and Pomona Valley
- Fort Worth Transportation Authority – Fort Worth
- Golden Gate Bridge, Highway and Transportation District – San Francisco
- Hillsborough Area Regional Transit Authority – Tampa
- Long Beach Transit – Long Beach
- Los Angeles County Metropolitan Transportation Authority – Los Angeles
- Memphis Area Transit Authority – Memphis
- Metropolitan Atlanta Rapid Transit Authority – Atlanta
- Metropolitan Transit Authority of Harris County – Houston
- Miami-Dade Transit – Miami
- Orange County Transportation Authority – Orange
- Pierce County Transportation Benefit Area Authority – Tacoma
- Regional Transportation Authority – Chicago
- Rochester-Genesee Regional Transportation Authority – Rochester
- RTC of Southern Nevada – Las Vegas
- San Diego Metropolitan Transit System – San Diego
- San Joaquin Regional Transit District – Stockton
- Texas A&M Transportation Institute – College Station
- Tri-County Metropolitan Transportation District of Oregon – Portland
- Utah Transit Authority – Salt Lake City
- Valley Metro Rail, Inc. – Phoenix
- VIA Metropolitan Transit – San Antonio
- Washington Metropolitan Area Transit Authority – Washington
